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qPCR is fairly very simple to complete in comparison with circulation cytometry that needs considerable know-how for reproducible benefits. Actually, move cytometric enumeration of microbial cells was originally restricted to pure cultures [31] and continue to remains tough when executed in elaborate matrices [32].

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The results were being correlated into the NGS-primarily based abundance of your dominant butyrate-developing genera Subdoligranulum

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Here, we explain how quantitative PCR (qPCR) accomplished in parallel to NGS library preparation offers an exact estimation of absolute taxon abundances from NGS knowledge and consequently delivers an attainable solution to compositionality in high-throughput microbiome analyses. The advantages and potential worries of the strategy can also be talked over.

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